Calcium Imaging

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Instrumentation

 

Calcium Imaging

Visitron-VisiFluor-montage large.jpg

     

VisiFluor High Performance Ca++ or FRET Imaging 

scientific grade cooled CCD cameras represent the state-of-the-art detector for spatially resolved calcium-measurements.

An integrated measurement system demands three essentials:

 

  • high sensitivity / low noise image sensors
  • fast, but accurate wavelength toggling
  • perfect synchronization of both components  

     

  Experimental Methods:

 

  • Fast change of excitation wavelength via polychromator or filterwheel
  • Excitation wavelength e.g. Fura-2
  • Emission wavelength, e.g. BCECF or CFP/YFP/FRET
  • Acquisition of two different emission bands via filterwheel (sequentially) or with our simultaneously with DualView Imager e.g. GFP-FRET or Indo.
  • Observation of single wavelength fluorescence of calcium-indicators
  • e.g. Fluo 3, Fluo 4

pdf VisiFluor_Calcium_englisch_05-2009a 364.56 Kb