FRAP Fluorescent Recovery After Photobleaching

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Instrumentation

 

FRAP

 

Visitron-FRAP-system large.jpg

 

All fluorescent dyes emit light of one wavelength after they have absorbed light of another wavelength. If a high intensity e.g. UV light illuminates the dyes, they react with photobleaching. The high intense light renders the dyes unable to emit fluorescence. FRAP is working based on this phenomenon and is used typically to measure the dynamics of molecular mobility or movement of fluorescent labeled molecules. It is also possible to measure the exchange of molecules between separate compartments of the cells. 

 

1D VisiFRAP System

 

  • Single point FRAP with about < 1,5 μm point resolution at 100x objective with high N.A.
  • Optimised optics for high photon efficiency
  • Choice of single or multiple wavelengths and laser lines
  • Parallel use of epi, TIRF or FRAP illumination
  • Easy and fast positioning manually or by a motorized xy stage
  • Fully software integration of high speed AOTF system for laser line selection


2D VisiFRAP System

 

  • Galvanometer controlled 2D module for illumination of independent multiple points or multiple ROI`s
  • Optimised optics for high photon efficiency
  • Choice of single or multiple wavelength and laser lines
  • High speed switching between realtime confocal CSU spinning disk
  • Point size of laser spot about 1um for 100x objective with high aperture of > 1.33 NA
  • FC fibre connector for laser system
  • Including Visitron FRAP software module


Typical Applications

 

  • 1D and 2D FRAP for cell biology
  • FLIP
  • fluorescent labeled macromolecules
  • Photoactivation
  • Acceptor photobleaching
  • Photoconversion studies

 

pdf VS_1D_VisiFRAP_2009 111.27 Kb

pdf VS_2D_VisiFRAP_2009_VisiView 244.95 Kb