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Life Sciences Instrumentation TIRF Total Internal Reflection Fluorescence 
TIRF Total Internal Reflection Fluorescence |
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Instrumentation
Visitron TIRF system
The TIRF technique is a special kind of wide-field fluorescence technique for measuring cells or SMC in an evanescent field without haze. By total reflection of the excited light (typically by laser) at the coverslip / medium interface, the fluorescence excitation is limited to a very thin space in the vicinity of the glass surface. The resulting fluorescence images exhibit extremely high contrast and resolution with the possibility of realtime imaging of cell membrane or labelled single molecules SMC.
The VisiTIRF Fluorescence Imaging System from Visitron Systems is the ideal solution for the analysis of finest structures or surfaces. The flexibility of the VisiTIRF system and the use of scientific digital CCD camera systems with optimal resolution, speed and sensitivity allows for the adaptation of customized application and configuration. For fluorescence illumination Visitron use solid state lasers or laser diodes . The coupling of the laser into the microscope is achieved by a flexible optical fibre. Visitron use a special self developed VisiTIRF condenser which allows for an easy adjustment of the laser beam to the correct illumination angle in the high aperture of the TIRF objective. A second optical fibre input allows for the combination of TIRF and epi fluorescence without moving any parts of the condenser. For epi fluorescence illumination Visitron offer a standard HBO / HXP lamp or the Visitron VisiChrome polychromator system
VisiTIRF System Components Overview
 Visitirf englisch 2009 sm 131.48 Kb |
